Sperm injected to women

This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal uterus. With this method, the acrosome reaction is skipped. However, the steps to be followed before and after insemination are the same. This is because the acrosome reaction has to take place and thousands of sperm cells have to be involved in IVF. Once fertilized, the egg is transformed into a proembryo and it has to be transferred to the uterus to continue its development.
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What is Intracytoplasmic Sperm Injection (ICSI)?

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FAQ: Intracytoplasmic Sperm Injection | UCSF Health

Once attached, the sperm pushes through the outer layer to the inside of the egg cytoplasm , where fertilization takes place. Sometimes the sperm cannot penetrate the outer layer, for a variety of reasons. In these cases, a procedure called intracytoplasmic sperm injection ICSI can be done along with in vitro fertilization IVF to help fertilize the egg. During ICSI, a single sperm is injected directly into the cytoplasm the egg.
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Intracytoplasmic Sperm Injection: ICSI

Intracytoplasmic sperm injection, or ICSI, involves injecting a single live sperm directly into the center of a human egg. The technique was developed to help achieve fertilization for couples with severe male factor infertility or couples who have had failure to fertilize in a previous in vitro fertilization IVF attempt. The procedure overcomes many of the barriers to fertilization and allows couples with little hope of achieving successful pregnancy to obtain fertilized embryos. The procedure requires that the female partner undergo ovarian stimulation with fertility medications so that several mature eggs develop. These eggs are then aspirated through the vagina using vaginal ultrasound, and incubated under precise conditions in the embryology laboratory.
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It is used when a male factor affects fertility. Through ICSI, a single sperm is injected directly into the egg. The sperm therefore bypasses several steps in the early fertilization process. In our on-site fertility lab , we are able to separate and look at individual sperm from a semen sample.
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